August 2020 Newsletter

"One to Watch" in the 
Nature Spinoff Prize 2020

TRANSFECTION REAGENTS FOR LEUKAEMIC CELLS

Among our host of nucleic acid delivery systems, we at RJH Biosciences have created effective transfection reagents specifically for attachment independent (suspension-growing) cells. As a synthetic amphiphilic polymer, Leu-Fect reagents were originally designed as siRNA transfection reagents. Further studies have proven Leu-Fect to be effective on adherent cells as well as for delivery of other types of nucleic acids. 

Reducing Colony Formation by Drug-Resistant K562 Cells Using Bcr-Abl siRNA and Proprietary microRNA (miR)

Inhibiting Gene Expression with siRNA Delivery using Leu-Fect A in RS4:11, KG1A, and K562 Leukemia Cells

Reducing Colony Formation with Specific siRNAs in Primary Patient Cells Ex Vivo

Transfecting K562 Cells with GFP mRNA/pDNA using Lipofection and mRNA-Fect

CONSIDERATIONS FOR OPTIMIZING NON-VIRAL TRANSFECTIONS IN CULTURE

The efficiency of all transfection reagents depends on the cell type to be modified, nature of polynucleotide to be delivered, and cell culture conditions. The transfection conditions for each cell type should be optimized to ensure maximal gene expression (or silencing) while minimizing cellular toxicity. We have complied a list of factors that should be considered for optimum transfection efficacy, which can be accessed here.

WE ARE HAPPY TO HELP!

Get personalized advice by an RJH representative on the best transfection methods for a specific application. We look forward to working with you soon.