"One to Watch" in the
Nature Spinoff Prize 2020
SCREENING SERVICE FOR OPTIMAL TRANSFECTION REAGENTS FOR YOUR CELLS
When it comes to transfecting a particular cell type, it is imperative to identify the right reagent that effectively delivers nucleic acid to cells. No two cell types are alike; each cell displays different proliferation rate, endocytosis activity and surface features that affect the efficiency of the reagents. Labour-intensive screening studies are often needed to understand which reagent is optimal in a particular cell type.
The RJH Biosciences offers libraries of transfection reagents that can be effective in almost all cell types. The reagents can be formulated for delivery of pDNA, mRNA, miR, siRNA, ASO and other nucleic acids. We now provide a service to help our customers to identify the right reagents for their application. This is cost-effective, time-saving approach that can pinpoint optimal reagent for your cells.
Transfecting Lung Cancer Cells with pDNA and Tailored RJH Reagents
Identifying Differential Reagents Effective for siRNA Delivery
CONSIDERATIONS FOR OPTIMIZING NON-VIRAL TRANSFECTIONS IN CULTURE
The efficiency of all transfection reagents depends on cell type to be modified, nature of polynucleotide to be delivered, and cell culture conditions. The transfection conditions for each cell type should be optimized to ensure maximal gene expression (or silencing) while minimizing cellular toxicity. We outlined our optimal procedure to transfect suspension-growing cells, which can be accessed here.
Transfecting PMSC with RJH Reagents and Other Commercial Reagents
Take a look at our latest application note focusing on transfecting peripheral blood mononuclear cells (PBMC) and other suspension growing cells with a mRNA-Fect. See the link here: http://www.rjhbiosciences.com/wp-content/uploads/2020/10/Application-Note-PBMC-mRNA.pdf
WE ARE HAPPY TO HELP!
Get personalized advice by an RJH representative on the best transfection methods for a specific application. We look forward to working with you soon.