December 2020 Newsletter

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"One to Watch" in the 
Nature Spinoff Prize 2020



SCREENING SERVICE FOR OPTIMAL TRANSFECTION REAGENTS FOR YOUR CELLS

When it comes to transfecting a particular cell type, it is imperative to identify the right reagent that effectively delivers nucleic acid to cells. No two cell types are alike; each cell displays different proliferation rate, endocytosis activity and surface features that affect the efficiency of the reagents. Labour-intensive screening studies are often needed to understand which reagent is optimal in a particular cell type.

The RJH Biosciences offers libraries of transfection reagents that can be effective in almost all cell types. The reagents can be formulated for delivery of pDNA, mRNA, miR, siRNA, ASO and other nucleic acids. We now provide a service to help our customers to identify the right reagents for their application. This is cost-effective, time-saving approach that can pinpoint optimal reagent for your cells.


Transfecting Lung Cancer Cells with pDNA and Tailored RJH Reagents

Transfecting lung cancer A549 cells with pDNA expressing GFP. The GFP expression was determined after 2 days of transfection. A small library of RJH reagents (71) was formulated with pDNA and exposed to the cells under optimal conditions. A variation in transfection efficiency was evident depending on the formulation details of the reagents.

Identifying Differential Reagents Effective for siRNA Delivery

Relative uptake of siRNA in VERO (African green monkey kidney epithelial) and MRC-7 (human fetal lung epithelial) cells after delivery with a library of RJH reagents. The cells were exposed to siRNAs for 24 hours and uptake was determined by flow cytometry. Some reagents were effective in both cell types while others were selective for each cell type.

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CONSIDERATIONS FOR OPTIMIZING NON-VIRAL TRANSFECTIONS IN CULTURE

The efficiency of all transfection reagents depends on cell type to be modified, nature of polynucleotide to be delivered, and cell culture conditions. The transfection conditions for each cell type should be optimized to ensure maximal gene expression (or silencing) while minimizing cellular toxicity. We outlined our optimal procedure to transfect suspension-growing cells, which can be accessed here.




Transfecting BMSC with RJH Reagents and Other Commercial Reagents

Typical transfections of PBMC with ALL-Fect reagent. The cells (from 2 sources) were transfected with a GFP expressing pDNA and GFP expression was visualized after 2 days with a fluorescence microscope. As comparison, leading lippofection and polymer mediated reagents are included under optimal conditions for each reagent.


Take a look at our latest application note focussing on transfecting peripheral blood mononuclear cells (PBMC) and other suspension growing cells with a mRNA-Fect. See the link here: http://www.rjhbiosciences.com/wp-content/uploads/2020/10/Application-Note-PBMC-mRNA.pdf


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WE ARE HAPPY TO HELP!

Get personalized advice by an RJH representative on the best transfection methods for a specific application. We look forward to working with you soon.


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