"One to Watch" in the
Nature Spinoff Prize 2020
USE OF RJH TRANSFECTION REAGENTS IN siRNA LIBRARY SCREENS
Having the ability to screen large numbers of compounds in one attempt enables head-to-head comparisons in an expeditious manner. One has to rely on a reproducible procedure and delivery that are similar for each member of the library component. This is especially the case when transfection reagents are used and robust performance is expected for the delivery of each library member. Work with large libraries also poses a cost burden that has to be minimized for long term utility of such screens.
The RJH transfection reagents are particularly suitable for screening nucleic acid libraries. They are provided in homogenous solutions that minimize well-to-well variations during screens. They can be formulated to function in different formats, either delivering library members on their own or in combination with other reagents for synergistic activities. They are also cost-effective to reduce the overall costs of the screens.
Library Screen in Normal Fibroblasts and Transformed Cells
Library Screen in Leukemic Cells
Library Screen in Breast Cancer Cells in Combination with TRAIL Protein
CONSIDERATIONS FOR OPTIMIZING NON-VIRAL TRANSFECTIONS IN CULTURE
The efficiency of all transfection reagents depends on cell type to be modified, nature of polynucleotide to be delivered, and cell culture conditions. The transfection conditions for each cell type should be optimized to ensure maximal gene expression (or silencing) while minimizing cellular toxicity. We have complied a list of factors that should be considered for optimum transfection, which can be accessed here.
Take a look at 'Electrospun gelatine matrices with bioactive pDNA polyplexes', a 2020 publication highlighting RJH Product "All-Fect" for electrospun matrices suitable for implantation.
WE ARE HAPPY TO HELP!
Get personalized advice by an RJH representative on the best transfection methods for a specific application. We look forward to working with you soon.