Order Reagents
All-Fect:
siRNA pDNA Transfection Reagent
$164.00 – $295.00 USD
- Transient transfection with pDNA, siRNA and co-delivery
- Attachment dependent and independent cells
- Stem, and primary cells
All-Fect: Versatile siRNA pDNA Transfection Reagent
All-Fect is a highly effective, broadly-acting gene delivery system useful in suspension cell transfection and It has been tailored as a siRNA pDNA cotransfection reagent for a variety of cell lines and is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Al-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. All-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent making it an excellent choice for transient transfection applications. All-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension cells. As with all transfection reagents, formulations of All-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions. Learn More
Mesenchymal Stem Cell Transfection with All-Fect
Transfection of Suspension Cells with All-Fect
Breast Cancer Cell Transfection
Overview:
| Property | All-Fect |
| Nucleic Acid | pDNA, siRNA |
| Cell Types | Common, Stem, Primary, Suspension |
| Transfection Type | Transient, Co-delivery |
| High Throughput | Compatible |
| Serum | Compatible |
| Buffer | Aqueous |
| Storage Temperature | -20ºC |
| Shelf Life at -20ºC | ~1 year |
Transfections per 1 mL of All-Fect
| Plate Size | Number of Transfections |
|---|---|
| 6-well (1200 µL/well) | 80 – 240 |
| 24-well (800 µL/well) | 125 – 250 |
| 48-well (400 µL/well) | 250 – 500 |
| 96-well (200 µL/well) | 500 – 1000 |
Validated Cell Types:
| pDNA | siRNA | Co-Delivery |
| Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) | Chronic Myeloid Leukemia Cells (K562) | Breast Cancer Cells (MDA-231) |
| Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) | Acute Myeloid Leukemia Cells (THP1) | |
| Kidney Fibroblast Cells (293-T) | Breast Cancer Cells (MDA-231) | |
| Breast Cancer Cells (MDA-231) | ||
| Breast Cancer Cells (MCF-7) | ||
| Human Lymphoma Cells (U-937) |