Transfection Reagents

All-Fect:
siRNA pDNA Transfection Reagent

$118.00 – $214.00 CAD

  • Transient transfection with pDNA, siRNA and co-delivery
  • Attachment dependent and independent cells
  • Stem, and primary cells
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All-Fect: Versatile siRNA pDNA Transfection Reagent

All-Fect is a highly effective, broadly-acting gene delivery system useful in suspension cell transfection and It has been tailored as a siRNA pDNA cotransfection reagent for a variety of cell lines and is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Al-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. All-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent making it an excellent choice for transient transfection applications. All-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension cells. As with all transfection reagents, formulations of All-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions. Learn More

All-Fect pDNA Cord Stem 2

Mesenchymal Stem Cell Transfection with All-Fect

pDNA delivery of GFP plasmid to cord-blood derived mesenchymal stem cells with Lipofectamine and All-Fect
All-Fect K562

Transfection of Suspension Cells with All-Fect

Transfecting attachment-independent K562 cells with plasmid DNA and siRNA using ALL-Fect and a leading lipofection reagent. (Left) GFP expression was induced with plasmid DNA and analyzed by flow cytometry 2 days after transfection. (Right) Survivin silencing and resultant inhibition of cell growth with specific siRNA 3 days after transfection.
All-Fect MD MDA 231

Breast Cancer Cell Transfection

Transfecting attachment-dependent MDA-MB-231 cells with plasmid DNA and siRNA using ALL-Fect and a leading lipofection reagent. (Left) GFP expression was induced with plasmid DNA and analyzed by fluorescent microscopy (insert) and flow cytometry 2 days after transfection. (Right) GFP silencing was induced with siRNA and analyzed by flow cytometry 2 days after transfection.

Overview:

Property All-Fect
Nucleic Acid pDNA, siRNA
Cell Types Common, Stem, Primary, Suspension
Transfection Type Transient, Co-delivery
High Throughput Compatible
Serum Compatible
Buffer Aqueous
Storage Temperature -20ºC
Shelf Life at -20ºC ~1 year

Transfections per 1  mL of All-Fect

Plate Size Number of Transfections
6-well (1200 µL/well) 80 – 240
24-well (800 µL/well) 125 – 250
48-well (400 µL/well) 250 – 500
96-well (200 µL/well) 500 – 1000

Validated Cell Types:

pDNA siRNA Co-Delivery
Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) Chronic Myeloid Leukemia Cells (K562) Breast Cancer Cells (MDA-231)
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) Acute Myeloid Leukemia Cells (THP1)
Kidney Fibroblast Cells (293-T) Breast Cancer Cells (MDA-231)
Breast Cancer Cells (MDA-231)
Breast Cancer Cells (MCF-7)
Human Lymphoma Cells (U-937)

 

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