$164.00 – $295.00 USD
All-Fect is a highly effective, broadly-acting gene delivery system useful in suspension cell transfection and It has been tailored as a siRNA pDNA cotransfection reagent for a variety of cell lines and is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Al-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. All-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent making it an excellent choice for transient transfection applications. All-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension cells. As with all transfection reagents, formulations of All-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions. Learn More
Property | All-Fect |
Nucleic Acid | pDNA, siRNA |
Cell Types | Common, Stem, Primary, Suspension |
Transfection Type | Transient, Co-delivery |
High Throughput | Compatible |
Serum | Compatible |
Buffer | Aqueous |
Storage Temperature | -20ºC |
Shelf Life at -20ºC | ~1 year |
Plate Size | Number of Transfections |
---|---|
6-well (1200 µL/well) | 80 – 240 |
24-well (800 µL/well) | 125 – 250 |
48-well (400 µL/well) | 250 – 500 |
96-well (200 µL/well) | 500 – 1000 |
pDNA | siRNA | Co-Delivery |
Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) | Chronic Myeloid Leukemia Cells (K562) | Breast Cancer Cells (MDA-231) |
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) | Acute Myeloid Leukemia Cells (THP1) | |
Kidney Fibroblast Cells (293-T) | Breast Cancer Cells (MDA-231) | |
Breast Cancer Cells (MDA-231) | ||
Breast Cancer Cells (MCF-7) | ||
Human Lymphoma Cells (U-937) |