Transfection Reagents

Leu-Fect A & B:
Leukaemia Cell Transfection Reagents

$154.00$276.00 CAD

  • For attachment-independent (suspension-growing) cells
  • Each Leu-Fect formulations is specifically tailored for different types of suspension growing cells
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Leu-Fect A & B: Leukaemia Cell Transfection Reagents

Leu-Fect A and B are a highly effective transfection reagent for attachment independent (suspension-growing) cells. Leu-Fect A and B were originally designed as leukaemia cell transfection reagents but, have proven effective on other suspension growing cells and adherent cells. Leu-Fect is a synthetic amphiphilic polymer that was specifically tailored for siRNA delivery to leukaemia cells. It is capable of undergoing multivalent interactions with siRNA and encapsulating co-incubated siRNA molecules into 100 nm particles with a net positive charge. The complexation between the siRNA and Leu-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. Leu-Fect A and B are a non-integrating carrier of siRNA, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. Leu-Fect has been tested and found effective in different types of attachment-independent cells, but users are advised to test the efficacy of the reagent in their particular cell type in order to choose the right formulation for long-term use. As with all transfection reagents, formulation of Leu-Fect with siRNA may need to be optimized for specific cell types. Learn More

Leu-Fect B-K562-cell-growth

Cell Growth of K562 Suspension Cells with Leu-Fect B

Growth of K562 cells after control (scrambled) and BCR-ABL siRNA delivery by Leu-Fect-B 3 days after transfection.
Leu-Fect-B-K562-mRNA

K562 Suspension Cell Transfection with Leu-Fect B vs Lipofectamine

BCR-ABL mRNA analysis for cells treated with control and BCR-ABL siRNA 2 days after transfection. Growth of K562 cells after control (scrambled) and BCR-ABL siRNA delivery by Leu-Fect-B 3 days after transfection.

Overview:

Property Leu-Fect A Leu-Fect B
Nucleic Acid siRNA siRNA
Cell Types Suspension-growing Suspension-growing
Transfection Type Transient Transient
High Throughput Compatible Compatible
Serum Compatible Compatible
Buffer Aqueous Aqueous
Storage Temperature -20 ºC 4 ºC
Shelf Life at -20 ºC 1 year 1 year

 

Transfections per 1  mL of Leu-Fect A or B

Plate Size Number of Transfections
6-well (1200 µL/well) 80 – 240
24-well (800 µL/well) 125 – 250
48-well (400 µL/well) 250 – 500
96-well (200 µL/well) 500 – 1000

Validated Cell Types:

Leu-Fect A (siRNA Delivery) Leu-Fect B (siRNA Delivery)
Chronic myeloid leukaemia cells (K562) Acute myeloid leukaemia cells (THP1)
Mononuclear Cells (MNC) from CML patients Mononuclear Cells (MNC) from CML patients

References

Gul-Uludag et al. Leukemia Research (2014) 38: 1299–1308.
Landry et al. J. Controlled Release (2016) 224: 8-21.
Valencia-Serna et al. J. Controlled Release (2013) 172: 495-503.

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