Transfection Reagents

mRNA-Fect:
mRNA Transfection Reagent

$265.00$480.00 CAD

  • Attachment dependent such as MCF-7, MDA-MB-436, MDA-MB-231 cells, and suspension cell lines such as K562 and THP-1 cells
  • Optimized for mRNA transfection in transient protein expression
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mRNA-Fect: mRNA Transfection Reagent

mRNA-Fect is a highly effective transient transfection reagent optimized for mRNA delivery to both attachment-depended and suspension-growing cells. mRNA-Fect is a synthetic amphiphilic polymer that is tailored for mRNA delivery after extensive testing of amphiphilic polymer libraries. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated mRNA into ~100 nm particles with a net positive charge. The complexation between the mRNA and mRNA-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. mRNA-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with this reagent. mRNA-Fect has been tested and found effective for plasmid DNA and siRNA delivery as well as in certain cell types. As with all transfection reagents, formulations of mRNA-Fect with polynucleotides may need to be optimized for specific cell types and transfection conditions.

mRNA-adherent1000

Transfection of Attachment-dependent Cells with mRNA-Fect

Transfecting attachment-dependent MCF-7, MDA-MB-436, and MDA-MB-231 cells with mRNA-Fect. A mRNA coding for a reporter protein (Green Fluorescent Protein, GFP) was used to assess the efficiency of mRNA expression. Typical GFP expression levels were visualized under fluorescent microscopy (top pictures). The mRNA expression levels were quantified by flow cytometry 72 hours after transfection and summarized as the percentage of cells positive for GFP (bottom graph). For comparison a leading lipofection reagent was used according to the manufacture’s instructions.
mRNA-suspension1000

Transfection of Suspension-growing Cells with mRNA-Fect

Transfecting suspension K562 and THP-1 cells with mRNA-Fect. A mRNA coding for a reporter protein (Green Fluorescent Protein, GFP) was used to assess the efficiency of mRNA expression. Typical GFP expression levels were visualized under fluorescent microscopy (top pictures). The mRNA expression levels were quantified by flow cytometry 72 hours after transfection and summarized as the percentage of cells positive for GFP (bottom graph). For comparison a leading lipofection reagent was used according to the manufacture’s instructions.

Overview:

Property mRNA-Fect
Technology Polymeric
Nucleic Acid mRNA
Cell Types Suspension, Attachment
Transfection Type Transient
High Throughput Compatible
Serum Compatible
Buffer Aqueous
Storage Temperature -20 ºC
Shelf Life at -20 ºC ~1 year

Transfections per 1  mL of mRNA-Fect:

Plate Size Number of Transfections
6-well (1200 µL/well) 40 – 330
24-well (800 µL/well) 60 – 500
48-well (400 µL/well) 125 – 1000
96-well (200 µL/well) 250 – 2000

Validated Cell Types:

mRNA-Fect (mRNA Delivery)
Breast cancer cells (MDA-MB-436)
Breast cancer cells (MDA-MB-231)
Breast cancer cells (MCF-7)
Chronic myeloid leukemia cells (K562)
Acute myeloid leukemia cells (THP-1)

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