mRNA-Fect: mRNA Transfection Reagent
mRNA-Fect is a highly effective transient transfection reagent optimized for mRNA delivery to both attachment-depended and suspension-growing cells. mRNA-Fect is a synthetic amphiphilic polymer that is tailored for mRNA delivery after extensive testing of amphiphilic polymer libraries. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated mRNA into ~100 nm particles with a net positive charge. The complexation between the mRNA and mRNA-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. mRNA-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with this reagent. mRNA-Fect has been tested and found effective for plasmid DNA and siRNA delivery as well as in certain cell types. As with all transfection reagents, formulations of mRNA-Fect with polynucleotides may need to be optimized for specific cell types and transfection conditions.