Transfection Reagents

Transfection Agent Samples

$0.00 CAD

  • Try out two of our products to see how they work for you
  • Free samples of 2 x 200 µl each, just pay shipping
  • Mix and match your choice of any products
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Try two of our transfection agent samples on your cells and see how they can increase your nucleic delivery efficiency. RJH Biosciences researches and develops high efficiency, low-toxicity, nucleic acid delivery systems for in vitro and in vivo applications.

Our Products:

All-Fect: designed for pDNA, siRNA, and co-delivery to a wide variety of cell types. Useful in transfecting most adherent cells, and suspension growing cells. Learn More

Prime-Fect: tailored for pDNA and some siRNA delivery to primary cells. Effective on both adherent and non-adherent primary cells. Learn More

Leu-Fect A & B: specially designed for Leukaemia cells. The “A” formulation is tailored for pDNA delivery, and “B” formulation for siRNA silencing. Learn More

293-Fect: originally customized for protein production in 293-cells, it is very effective for pDNA delivery to fast growing cells. Excellent for protein production. Learn More

 

Product Specifications

Property All-Fect
Technology Polymeric
Nucleic Acid pDNA, siRNA
Cell Types Common, Stem, Primary, Suspension
Transfection Type Transient, Co-delivery
High Throughput Compatible
Serum Compatible
Buffer Aqueous
Storage Temperature -20ºC – 4ºC
Shelf Life at -20ºC ~1 year
Property Prime-Fect
Technology Polymeric
Nucleic Acid pDNA, siRNA
Cell Types Primary (pDNA), and other cell lines (siRNA)
Transfection Type Transient
High Throughput Compatible
Serum Compatible
Buffer Aqueous
Storage Temperature -20ºC – 4ºC
Shelf Life at -20ºC ~1 year

 

Property Leu-Fect A Leu-Fect B
Technology Polymeric Polymeric
Nucleic Acid siRNA siRNA
Cell Types Suspension-growing Suspension-growing
Transfection Type Transient Transient
High Throughput Compatible Compatible
Serum Compatible Compatible
Buffer Aqueous Aqueous
Storage Temperature -20 ºC or 4 ºC -20 ºC or 4 ºC
Shelf Life at -20 ºC ~1 year ~1 year
Property 293-Fect
Technology Polymeric
Nucleic Acid pDNA
Cell Types 293T cells and other fast-growing cell lines
Transfection Type Transient
High Throughput Compatible
Serum Compatible
Buffer Aqueous
Storage Temperature -20 ºC – 4 ºC
Shelf Life at -20 ºC ~1 year

 

Transfections per 1  mL of RJH Transfection Reagents

Plate Size Number of Transfections
6-well (1200 µL/well) 80 – 240
24-well (800 µL/well) 125 – 250
48-well (400 µL/well) 250 – 500
96-well (200 µL/well) 500 – 1000

Published Cell Types

All-Fect Published Cell Lines

pDNA siRNA Co-Delivery
Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) Chronic Myeloid Leukemia Cells (K562) Breast Cancer Cells (MDA-231)
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) Acute Myeloid Leukemia Cells (THP1)
Kidney Fibroblast Cells (293-T) Breast Cancer Cells (MDA-231)
Breast Cancer Cells (MDA-231)
Breast Cancer Cells (MCF-7)
Human Lymphoma Cells (U-937)

 

Prime-Fect Published Cell Lines

<trwidth=”456″>pDNAUmbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC)Bone Marrow Derived Mesenchymal Steam Cells (BM-MSC)Vascular Smooth Muscle Cells (VSMCs)Kidney Fibroblast Cells (293-T)Breast Cancer Cells (MDA-231, MCF-7)Human Lymphoma Cells (U-937)

 

Leu-Fect Published Cell Lines

Leu-Fect A (siRNA Delivery)Leu-Fect B (siRNA Delivery)

Chronic myeloid leukaemia cells (K562) Acute myeloid leukaemia cells (THP1)
Mononuclear Cells (MNC) from CML patients Mononuclear Cells (MNC) from CML patients

 

293-Fect Published Cell Lines

pDNA Delivery
Kidney fibroblast cells (293-T)
Breast cancer cells (MDA-231)
Breast cancer cells (MCF-7)
Vascular Smooth Muscle Cells (VSMCs)
Human Umbilical Vein Endothelial Cells (HUVECs)
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC)
Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC)

Resources

SDS In Vitro Reagents

References and Testimonials

Hsu and Uludağ. Biomaterials (2012) 33: 7834-7848.
Remant Bahadur et al., J. Materials Chemistry B (2015) 3: 3972-3982.
Wang et al., J. Surgical Research (2013) 183: 8-17.

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