Transfection Reagents

Reagents for Your R&D Activity


We developed broadly acting transfection reagents to modify mammalian cells with plasmid DNA, siRNA, mRNA and other nucleic acids. The foundation of our transfection reagents is based on cationic lipopolymers with optimal balance of cationic charge and hydrophobic (lipid) group. By systematically altering the polymeric backbone and the nature of lipid group, a library of transfection reagents has been generated. Some of the transfection reagents are broadly acting, functioning in different cell types with different nucleic acids. Others display high specificity, where they are exceptionally effective in particular cell types for particular nucleic acid delivery.

The transfection reagents have been optimized for the following cell models and applications:

Cell Lines in Culture:

  • kidney fibroblast cells (293T) and epitheliam cells (MDCK)
  • breast cancer cells (MDA-MB-231, MDA-MB-468, MDA-MB-436, SUM-149PT, MCF-7)
  • chronic myeloid leukemia cells (K562)
  • acute myeloid leukemia cells (THP-1, KG1, KG1A)
  • acute lymphocytic leukemia cells (NALM-6 and MOLM-13)
  • lymphoma cells (U937)
  • lung cancer cells (A549)
  • colon cancer cells (HCT-116)
  • immortalized myoblast cells
  • Primary Cells in Culture:

  • primary mononuclear cells from blood and bone marrow
  • skin fibroblasts
  • vascular smooth muscle cells
  • endothelial cells
  • mesenchymal stem cells (human and rodent derived)
  • Animal models:

  • systemic and local injection of RNAi mediator siRNA
  • local injection of pDNA and mRNA expression vectors
  • For a classification our transfection reagents by cell type, please see our Transfection Reagent Selection Guide.

    For a detailed description of our transfection reagents please see below. Once you decide the right transfection reagent for your application, please proceed to our Store to place an order. We provide 50% discount for first-time customers.

    For questions about products and their use, please contact us at rjhbiosciences@gmail.com



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