Transfection Reagents

Reagents for Your R&D Activity


We developed broadly acting transfection reagents to modify mammalian cells with plasmid DNA, siRNA, mRNA and other nucleic acids. The foundation of our transfection reagents is based on cationic lipopolymers with optimal balance of cationic charge and hydrophobic (lipid) group. By systematically altering the polymeric backbone and the nature of lipid group, a library of transfection reagents has been generated. Some of the transfection reagents are broadly acting, functioning in different cell types with different nucleic acids. Others display high specificity, where they are exceptionally effective in particular cell types for particular nucleic acid delivery.

The key advantages of our delivery vehicles are:

transfection process of lipopolymers with a list of benefits according to each stage of transfection

The transfection reagents have been optimized for the following cell models and applications:

Primary Cells

Attachment Dependent

  • VSMC (Vascular Smooth Muscle Cells)
  • HUVEC (Human Umbilical Vein Endothelial Cells)
  • Human Foreskin Fibroblast Cells
  • BMSC (Bone Marrow Stromal Cells)
  • Human Myoblasts
  • Rat Primary Sympathetic Neurons

Attachment Independent

  • UCB-MSC (Umbilical Cord Blood Derived Mesenchymal Stem Cells)
  • BM-MSC (Bone Marrow Derived Mesenchymal Stem Cells)
  • Mononuclear Cells from Leukemia Patients
  • Mononuclear Cells from Normal Human Blood
Cell Lines

Attachment Dependent

  • 293-T (Kidney Fibroblast Cells)
  • MDA-231 (Breast Cancer Cells)
  • MDA-436 (Breast Cancer/ Melanoma Cells)
  • MDA-468, Sum-149PT, MCF-7 (Breast Cancer Cells)
  • A549 (Human Lung Cancer Cells)
  • MDCK (Kidney Epithelial Cells)
  • HCT-116 (Human Colon Cancer Cells)
  • C2C12 Myoblast Cells
  • MC3T3-1 (Preosteoblast Cells)
  • Green Monkey Vero Cells

Attachment Independent

  • U-937 (Human Lymphoma Cells)
  • K562 (Chronic Myeloid Leukemia Cells)
  • KG1, KG1A and THP-1 (Acute Myeloid Leukemia Cells)
  • Jurkat T-Cells
Animal Models
  • Systemic and local injection of RNAi mediator siRNA
  • Local injection of pDNA and mRNA expression vectors

For a classification our transfection reagents by cell type, please see our Transfection Reagent Selection Guide.

For a detailed description of our transfection reagents please see our summary of reagents page. Once you decide the right transfection reagent for your application, please proceed to our Store to place an order. We provide 50% discount for first-time customers.

Using a cell type or model that's not on our list? We now offer screening services that can help find the best RJH reagent for your system

For questions about products and their use, please contact us at rjhbiosciences@gmail.com