September 2020 Newsletter

"One to Watch" in the
Nature Spinoff Prize 2020

TRANSFECTION REAGENTS FOR ANIMAL MODELS

Among our host of nucleic acid delivery systems, we created two highly effective transfection reagents for use in animal studies:
In Vivo DNA-Fect and In Vivo RNA-Fect.

In Vivo DNA-Fect is a synthetic amphiphilic polymer that is specifically tailored for plasmid DNA delivery. It has been tested and found effective in different types of primary attachment dependent cells as well as for cell culture use.

In Vivo RNA-Fect is a synthetic amphiphilic polymer that is specifically tailored for siRNA delivery. It has been tested and found effective after administration via systemic routes, such as intravenous and intraperitoneal, as well as local administration.

Delivery of pDNA using In Vivo DNA-Fect and PEI

delivery of pDNA using DNA-Fect transfection reagent — Two different GFP expression plasmids (EGFP and AcGFP) were used for subcutaneous implantation in rats. The delivery agents were either 25 kDa branched PEI (top 2 pictures) or In Vivo DNA-Fect (bottom two pictures). The implants were recovered and visualized for GFP expression by epifluoresecent microscopy. GFP positive cells were primarily observed when **In Vivo DNA-Fect** was used for plasmid delivery (arrows).

Tumor Growth as a Result of siRNA Delivery with In Vivo RNA-Fect

siRNA delivery using in vivo RNA-fect transfection reagent — Subcutaneous MDA-MB-231 tumors were established in nu/nu mouse. siRNAs (controls and therapeutic siRNAs) formulated with **In Vivo RNA-Fect** were injected subcutaneously **(left)** and tumor growth was assessed by external measurements. siRNAs (control and therapeutic siRNAs) formulated with **In Vivo RNA-Fect** were also injected intraperitoneally **(right)** and tumor growth was assessed.

CONSIDERATIONS FOR OPTIMIZING NON-VIRAL TRANSFECTIONS IN CULTURE

The efficiency of all transfection reagents depends on cell type to be modified, nature of polynucleotide to be delivered, and cell culture conditions. The transfection conditions for each cell type should be optimized to ensure maximal gene expression (or silencing) while minimizing cellular toxicity. We have complied a list of factors that should be considered for optimum transfection, which can be accessed here.

Take a look at 'Electrospun gelatine matrices with bioactive pDNA polyplexes', a 2020 publication highlighting RJH Product "All-Fect" for electrospun matrices suitable for implantation.

All-Fect transfection reagent from RJH Biosciences

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Get personalized advice by an RJH representative on the best transfection methods for a specific application. We look forward to working with you soon.

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