Summary of Reagents

All-Fect

Designed as a siRNA pDNA transfection reagent it can be used for either siRNA knockdown, plasmid DNA transformation or siRNA pDNA co-delivery. It is particularly suitable for mesenchymal stem cells from cord blood and bone marrow, and highly differentiated cells such as smooth muscle cells, and endothelial cells.

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Benefits of the reagent :
  • High transfection efficiency
    Provides 2 to 3-fold higher efficacy in the presences of serum
  • Simple Protocol
    No need to change tissue culture medium during transfection
  • Toxicity
    Less toxic compared to commercial transfection reagents, leading to better retention of normal cellular physiology
References :
  • Hsu and Uludağ. Biomaterials (2012) 33: 7834-7848.
  • Remant Bahadur et al., J. Materials Chemistry B (2015) 3: 3972-3982.
  • Wang et al., J. Surgical Research (2013) 183: 8-17.


Typical performance of All-Fect for transfecting cord-blood derived mesenchymal stem cells with a GFP plasmid. The study compared the performance of All-Fect against a leading lipofection reagent under conditions optimized for each reagent. The extent of transgene expression was quantitated by flow cytometry, based on the extent of EGFP expression in arbitrary units. Typical fluorescent micrographs showing GFP expression after transfection are provided on the top.

Prime-Fect

This product is designed for transfection of primary cells with plasmid DNA. Prime-Fect has been tailored for attachment dependant cell plasmid DNA delivery, but also has been effective in siRNA delivery to attachment dependant cells and plasmid transfection of non-attachment dependant cells.

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Benefits of the reagent :
  • High transfection efficiency on primary cells
    Provides 2 to 3-fold higher efficacy in the presences of serum
  • Simple Protocol
    No need to change tissue culture medium during transfection
  • Toxicity
    Less toxic compared to lipofection reagents, leading to minimal alteration of cell physiology
References :
  • KC et al., J. Materials Chemistry B (2015) 3: 3972-3982.


Transfecting bone marrow stromal cells with Prime-Fect. GFP expression was induced with a plasmid and analyzed by fluorescent microscopy 2 days after transfection. Cell-associated DNA is visualized with a red label (A). Transfecting breast cancer MDA-MB-231 and MCF-7 cells with siRNA using Prime-Fect. The expression levels of two target genes were analyzed by qPCR after delivery of control (scrambled) and gene-specific siRNAs (2 days after transfection) (B). Transfecting umbilical cord-derived mesenchymal stem cells with Prime-Fect. GFP expression was induced with a plasmid DNA and analyzed by fluorescent microscopy 2 days after transfection. (C) A leading polymeric transfection reagent and (D) Prime-Fect.>

Leu-Fect A & B

These products are designed for transfection of attachment-independent (suspension growing) cells and are specifically tailored for siRNA delivery. We offer two different formulations (Leu-Fect A and Leu Fect B) that are effective in different types of attachment-independent cells. We recommend the users to test the efficacy of both reagents in their particular cell type and choose the right formulation for long-term use.

Download Information Leu-Fect A Brochure
Leu-Fect B Brochure
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Benefits of the reagent :
  • High transfection efficiency
    Provides 2 to 3-fold higher efficacy in the presences of serum
  • Simple Protocol
    No need to change tissue culture medium during transfection
  • Toxicity
    Less toxic compared to lipofection reagents, leading to minimal alteration of cell physiology
References :
  • Gul-Uludag et al. Leukemia Research (2014) 38: 1299–1308.
  • Landry et al. J. Controlled Release (2016) 224: 8-21.
  • Valencia-Serna et al. J. Controlled Release (2013) 172: 495-503.



Uptake of Leu-Fect/FAM-siRNA complexes in K562 cells in vitro (A) and in vivo K562 tumors (B). Typical performance of Leu-Fect for transfecting K562 chronic myeloid leukemia (CML) cells with a specific siRNA against Bcr-Abl. The study compared the performance of Leu-Fect against a leading lipofection reagent and branched PEI (PEI25; 25 kDa) under conditions optimized for each reagent. The extent of gene silencing was quantitated by qPCR (C). The functional outcome, in the form of inhibition of cell growth, was assessed by the MTT Assay and expressed relative to non-treated cells (D).

293-Fect

This product is designed for transfection of attachment dependent and rapidly dividing cells 293T cells with plasmid DNA. These cells are routinely transfected for small scale production of proteins/viruses for R&D purposes, as well as industrial scale production of proteins for commercial and clinical uses. This reagent is also effective with other attachment-dependent cells such as CHO cells and breast cancer MDA-MB-231 cells.

Download Infromation 293-Fect Brochure
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Benefits of the reagent :
  • High transfection efficiency
    Provides 2 to 3-fold higher efficacy in the presences of serum
  • Simple Protocol
    No need to change tissue culture medium during transfection
  • Toxicity
    Less toxic compared to lipofection and polymeric reagents, leading to better protein yields from the transfected cells
  • Stability
    Long term stability at room temperature
References :
  • Clements et al., Clinical Orthopeadics and Related Research (2009) 467: 3129-3137.
  • Naemnark et al., Molecular Pharmaceutics (2009) 6: 1798-1815.




Typical performance of 293-Fect for transfecting attachment-dependent 293 cells with a GFP plasmid is shown in the figure. The study compared the performance of 293-Fect against a leading lipofection reagent and linear PEI (L-PEI; 44 kDa) under conditions optimized for each reagent. Typical fluorescent micrographs showing EGFP expression after transfection (A). The extent of transgene expression was quantitated by flow cytometry, based on the percentage of cells expressing the GFP gene (B) and the extent of GFP expression in arbitrary units (C).

In Vivo DNA-Fect

This product is designed for delivery of plasmid DNA in animal models. It is a cost-effective alternative to PEI-based reagents marketed for in vivo applications, with improved transfection efficiency. The application of current reagents is usually limited by their toxicity, which either limits the expression of the transgenes or alters the physiological response in the vicinity of the administered agents. The biocompatibility of In Vivo DNA-Fect minimally alters the cellular physiology, allowing a better response of the expressed transgenes.

Download Information In Vivo DNA-Fect Brochure
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Benefits of the reagent :
  • High transfection efficiency
    Provides 2 to 3-fold higher efficacy under serum conditions
  • Simple Protocol
    Can be administered with simple formulations such as saline
  • Toxicity
    Less toxic compared to commercial transfecting reagents, leading to better protein yields from the transfected cells
References :
  • Rose et al., Biomaterials Science (2014) 2: 833-842.
  • Rose et al., Biomaterials (2012) 33: 3363-3374.



Typical performance of In Vivo DNA-Fect for transfecting tissues with two separate GFP plasmids at the rat subcutaneous site is shown in the figure. The study compared the performance of In Vivo DNA-Fect against branched PEI (PEI25; 25 kDa), after the corresponding complexes were implanted in gelatin sponges for 14 days. The extent of transgene expression was assessed by histology of explanted tissues, followed by the fluorescent microscopy.

In Vivo RNA-Fect

This product is designed for delivery of siRNA in mice tumor models. It can be directly administered into a specific site (e.g. intratumorally), or systematically (e.g. intraperitoneal, intravenous). It is a cost-effective alternative to PEI-based reagents marketed for in vivo applications. It's low toxicity allows for higher doses of nucleic acids to be administered without affecting the health of animal models.

Download Information In Vivo RNA-Fect Brochure
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Benefits of the reagent :
  • High transfection efficiency
    Provides effective transfection under physiological conditions
  • Simple Protocol
    siRNA particles can be prepared in saline suitable for administration
  • Toxicity
    Less toxic compared to other commercial transfecting reagents
References :
  • Aliabadi et al., J. Controlled Release (2013) 172: 219-228.
  • Alshamsan et al., Translational Oncology (2011) 4: 178-188.
  • Abbasi et al., Pharmaceutical Research (2011) 28: 2516-2529.


Tumor growth profiles as a result of siRNA delivery with In Vivo RNA-Fect is shown in the figure. The tumors were left untreated, or treated with scrambled and therapeutic siRNAs formulated with the transfection reagent. Formulations injected intratumorally (A). Formulations injected intraperitoneally (B).

mRNA-Fect

This product is designed for mRNA delivery of both attachment-dependent and attachment-independent (suspension growing) cells. mRNA-Fect has also been tested and found effective for plasmid DNA and siRNA delivery in certain cell types. We recommend the users to test the efficacy of the reagent in their particular cell type and choose the right formulation for long-term use.

Download Information mRNA-Fect Brochure
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Benefits of the reagent :
  • High transfection efficiency
    Provides 2 to 7-fold higher efficacy in the presences of serum compared with lipofection reagents
  • Simple Protocol
    No need to change tissue culture medium during transfection
  • Toxicity
    Less toxic compared to lipofection reagents, leading to minimal alteration of cell physiology



Transfection of mRNA with mRNA-Fect in (A) attachment-dependent MCF-7, MDA-MB-436 and MDA-MB-231 cells and (B) suspension-growing K562 and THP-1 cells. An mRNA coding for a reporter protein (Green Fluorescent Protein, GFP) was used to assess the efficiency of mRNA expression. Typical GFP expression levels were visualized under fluorescent microscopy (top pictures). The expression levels were quantitated by flow cytometry 72 hours after transfection and summarized as the percentage of cells positive for GFP (bottom graphs). For comparison, a leading lipofection reagent was used according to the manufacturer’s instructions.
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