The lanes treated with the scrambled siRNA (#2, 4, 9, 10, and 11) gave band intensities equivalent to the untreated cells (#1), irrespective of the transfection reagent used. With PNKP specific siRNA treatments, significant reductions in the band intensities were evident; LipofectamineTM 2000 at ratio of 1:4 was more potent than 1:2 (siRNA:reagent respectively). All 3 RJH reagents were effective in reducing the PKNP protein levels, indicating the versatility of the reagents. Note that highest silencing was seen with 293-Fect in this study. All 3 RJH reagents, ALL-Fect, 293-Fect and Prime-Fect were effective in silencing the chosen target by 80-90%, based on semi-quantitative densitometric analysis.
| Data courtesy of I. Paiva, University of Alberta (Canada)|