Western Blot analysis of breast cancer cells transfected with siRNA/Prime-Fect complexes. A specific siRNA against LPP1 (Lipid Phosphate Phosphohydrolase Type I) was formulated with Prime-Fect and added to the cells at 60 and 120 pmol (cells treated in 6-well plates; complexes prepared with 4.2 µL Prime-Fect and the indicated amount of siRNA). After 48 hours, the levels of the targeted protein was analysed by the western blotting. Note that a negative control (non-specific) siRNA did not affect the level of LPP1, while the protein levels were extinguished with the specific siRNAs delivered by using Prime-Fect. The loading was equivalent in each lane (lower gel).