Cells treated with the scrambled siRNA/RNAiMAX (Lane 1) served as the control band, where no specific silencing was presumed. Treatment with TP53 specific siRNA and LipofectamineTM RNAiMAX, CaCl2, Prime-Fect and 293-Fect showed effective reduction of the p53 protein levels. Highest transfection of A549 lung cancer cells was achieved with the Prime-Fect
(>75%, Lane 5) and RNAiMAX (~70%, Lane 2) based on semi-quantitative densitometric analysis. There were no obvious differences in B-actin loading onto the lanes among the samples (not shown). Simple screening of these transfection reagents led to the conclusion that Prime-Fect showed the highest effectiveness for lung cancer A549 cells.