Transfection Reagent Selection Guide

The table below summarizes optimal transfection reagents for nucleic acids in different cell types. The efficiency of the transfection reagents was assessed by using plasmid DNA (pDNA), short interfering RNA (siRNA), and messenger RNA (mRNA). Where the transfection reagent was suitable for both pDNA or siRNA delivery, it was indicated with pDNA/siRNA.

Cell TypeAll-FectTrans-BoosterLeu-Fect-ALeu-Fect-BPrime-FectmRNA-FectCRISP-Fect
Primary Cells Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) pDNA pDNA mRNApDNA
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) pDNApDNA mRNApDNA
Vascular smooth muscle Cells (VSMCs)pDNA mRNApDNA
Human Umbilical Vein Endothelial Cells (HUVECs)pDNA mRNA
Mononuclear Cells from CML patients (MNC)pDNA mRNAsiRNAsiRNA
Human Foreskin Fibroblast CellspDNA
Rat Primary Sympathetic NeuronspDNAmRNA
Cell LinesKidney Fibroblast Cells (293-T)pDNApDNA mRNApDNA
Breast Cancer Cells (MDA-MB-231)pDNA siRNA co-deliverypDNA mRNApDNAsiRNAmRNA
Kidney Epithelial Cells (MDCK)siRNA
Breast Cancer/Melanoma Cells (MDA-MB-436)pDNA mRNA siRNA mRNARNP
Breast Cancer Cells (MDA-MB-468)siRNA
Breast Cancer Cells (Sum-149PT)pDNA mRNAsiRNA
Breast Cancer Cells (MCF-7)pDNApDNA mRNApDNA siRNAmRNA
Human Lymphoma Cells (U-937)pDNApDNA
Chronic Myeloid Leukaemia Cells (K562)siRNApDNA mRNAsiRNAmRNA
Acute Myeloid Leukemia Cells (KG1 and KG1A)siRNAsiRNA
Acute Myeloid Leukemia Cells (THP1)siRNAsiRNAmRNA
Human Lung Cancer Cells (A549)siRNA
Human Colon Cancer (HCT-116)siRNAsiRNA
Human MyoblastsASO
Jurkat CellspDNAmRNARNP