The table below summarizes optimal transfection reagents for nucleic acids in different cell types. The efficiency of the transfection reagents was assessed by using plasmid DNA (pDNA), short interfering RNA (siRNA), and messenger RNA (mRNA). Where the transfection reagent was suitable for both pDNA or siRNA delivery, it was indicated with pDNA/siRNA.
Cell Type | All-Fect | Trans-Booster | Leu-Fect-A | Leu-Fect-B | Prime-Fect | mRNA-Fect | CRISP-Fect | |
---|---|---|---|---|---|---|---|---|
Primary Cells | Umbilical Cord Blood Derived Mesenchymal Stem Cells (UCB-MSC) | pDNA | pDNA mRNA | pDNA | ||||
Bone Marrow Derived Mesenchymal Stem Cells (BM-MSC) | pDNA | pDNA mRNA | pDNA | |||||
Vascular smooth muscle Cells (VSMCs) | pDNA mRNA | pDNA | ||||||
Human Umbilical Vein Endothelial Cells (HUVECs) | pDNA mRNA | |||||||
Mononuclear Cells from CML patients (MNC) | pDNA mRNA | siRNA | siRNA | |||||
Human Foreskin Fibroblast Cells | pDNA | |||||||
Rat Primary Sympathetic Neurons | pDNA | mRNA | ||||||
Cell Lines | Kidney Fibroblast Cells (293-T) | pDNA | pDNA mRNA | pDNA | ||||
Breast Cancer Cells (MDA-MB-231) | pDNA siRNA co-delivery | pDNA mRNA | pDNA | siRNA | mRNA | |||
Kidney Epithelial Cells (MDCK) | siRNA | |||||||
Breast Cancer/Melanoma Cells (MDA-MB-436) | pDNA mRNA | siRNA | mRNA | RNP | ||||
Breast Cancer Cells (MDA-MB-468) | siRNA | |||||||
Breast Cancer Cells (Sum-149PT) | pDNA mRNA | siRNA | ||||||
Breast Cancer Cells (MCF-7) | pDNA | pDNA mRNA | pDNA siRNA | mRNA | ||||
Human Lymphoma Cells (U-937) | pDNA | pDNA | ||||||
Chronic Myeloid Leukaemia Cells (K562) | siRNA | pDNA mRNA | siRNA | mRNA | ||||
Acute Myeloid Leukemia Cells (KG1 and KG1A) | siRNA | siRNA | ||||||
Acute Myeloid Leukemia Cells (THP1) | siRNA | siRNA | mRNA | |||||
Human Lung Cancer Cells (A549) | siRNA | |||||||
Human Colon Cancer (HCT-116) | siRNA | siRNA | ||||||
Human Myoblasts | ASO | |||||||
Jurkat Cells | pDNA | mRNA | RNP |
RJH manufactures a variety of transfection agents for your R&D needs. Download our catalogue for a list of our products.